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Fig. 4. A set of 15 genes change in opposite directions at the mRNA level in denervation and obstruction. The Venny analysis tool was used to identify genes that were increased in denervation (at Q 0.05) and reduced in obstruction (at Q 0.05) or vice versa. Fifteen mRNAs satisfied these criteria. Changes of these mRNAs are plotted in A–C. Italicized gene products met the Q 0 criterion, and the remainder met the Q 0.05 criterion. D: 4 examples of immunohistochemical stainings from the Human Protein Atlas for gene products in this set. Brown indicates positive staining. Antibodies for all of these targets stained the urothelial cell layer of the bladder. E: Western blots for <t>Wdr77</t> and Flot2 in denervation and obstruction.
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Fig. 4. A set of 15 genes change in opposite directions at the mRNA level in denervation and obstruction. The Venny analysis tool was used to identify genes that were increased in denervation (at Q 0.05) and reduced in obstruction (at Q 0.05) or vice versa. Fifteen mRNAs satisfied these criteria. Changes of these mRNAs are plotted in A–C. Italicized gene products met the Q 0 criterion, and the remainder met the Q 0.05 criterion. D: 4 examples of immunohistochemical stainings from the Human Protein Atlas for gene products in this set. Brown indicates positive staining. Antibodies for all of these targets stained the urothelial cell layer of the bladder. E: Western blots for Wdr77 and Flot2 in denervation and obstruction.

Journal: American journal of physiology. Renal physiology

Article Title: Array profiling reveals contribution of Cthrc1 to growth of the denervated rat urinary bladder.

doi: 10.1152/ajprenal.00499.2017

Figure Lengend Snippet: Fig. 4. A set of 15 genes change in opposite directions at the mRNA level in denervation and obstruction. The Venny analysis tool was used to identify genes that were increased in denervation (at Q 0.05) and reduced in obstruction (at Q 0.05) or vice versa. Fifteen mRNAs satisfied these criteria. Changes of these mRNAs are plotted in A–C. Italicized gene products met the Q 0 criterion, and the remainder met the Q 0.05 criterion. D: 4 examples of immunohistochemical stainings from the Human Protein Atlas for gene products in this set. Brown indicates positive staining. Antibodies for all of these targets stained the urothelial cell layer of the bladder. E: Western blots for Wdr77 and Flot2 in denervation and obstruction.

Article Snippet: Following one-dimensional (1D) separation and transfer to nitrocellulose, proteins were detected using the following antibodies: CTHRC1 (mmcri, vli55), PRC1 (no. 3639; Cell Signaling), PLOD2 (21214–1-AP; Proteintech), DKK3 (ab186409; Abcam), PERK (no. 3192; Cell Signaling), CAV1 (no. 3267; Cell Signaling), myosin phosphatase targeting subunit (MYPT/MYPT1, no. 2634; Cell Signaling), myosin light chain kinase (MYLK, M 7905; Sigma), pleiotrophin (PTN, ab79411; Abcam), gelsolin (GSN, no. 12953; Cell Signaling), osteopontin (SPP1, ab91665; Abcam), CD68 (MCA5709; Bio-Rad), WDR77 (D56B8, no. 2018S; Cell Signaling), FLOT2 (no. 3436S; Cell Signaling), -actin (A5441; Sigma Aldrich), and HSP90 (610418; BD Transduction Laboratories).

Techniques: Immunohistochemical staining, Staining, Western Blot